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Background: The superficial cutaneous mycoses are usually confined to the outer layers of skin, hair, and nails, and don’t invade living tissues. Dermatophytes and yeasts are the main pathogenic agents of these mycoses. In addition, some molds are mentioned as causative agents of these diseases. This study aims to identify the cutaneous fungal species from 181 dermatophytosis patients in Hospital of Hue University of Medicine and Pharmacy, Viet Nam. Methods: A cross sectional, descriptive survey on 181 dermatophytosis patients with positive direct examination from samples, including skin, hair and nail scrapings. These specimens were cultured on Sabouraud agar - Chloramphenicol medium or Sabouraud agar - Chloramphenicol - Cycloheximide medium. The culture tubes were incubated at 25 0C and examined daily for 4 weeks. Dermatophytes and nondermatophyte molds were identified by the microscopic morphology. Identification of Candida species and other yeast pathogens based on germ tube tests, cultivation on Corn Meal agar medium and colorimetric sugar utilization tests (Auxacolor test). Results: Dermatophytes was the most prevalent cutaneous fungal infection (90.64%), followed by yeasts (7.74%) and then non dermatophytes molds (1.65%). As the causative dermatophytes species, Trichophyton rubrum was the most frequently isolated pathogen (58.01%). T.rubrum and T.mentagrophytes were isolated from all the dermatophytosis clinical types. Candida sp and Trichosporon cutaneum were etiological agents of paronychia - onychomycosis. Fusarium sp. were pathogenic in onychomycosis and tinea corporis. Scopulariopsis sp. caused of onychomycosis. Conclusion: In this study, the cutaneous fungal pathogens were the highest rate of anthropophilic dermatophytes, and then zoophilic dermatophytes. Candida albicans and Candida non albicans were also found in paronychia - onychomycosis patients. In addition some nondermatophyte mold infections were found in both of skin and nail.

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