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Objective: The protist Trichomonas vaginalis is the most common non-viral, curable, sexually transmitted disease (STD) agent worldwide. The overall objective of this study is to evaluate the antibody response against T. vaginalis during follow-up visits of pharmacologically treated patients. Materials and methods: The study included 46 women affected by trichomoniasis and 8 male sexual partners. All women were subjected to standard clinical examination, vaginal samples were collected for identification of Trichomonas vaginalis by wet mount and cultivation in specific media. Serological reactivity of patients affected by trichomoniasis was studied for a 5 months period after pharmacological treatment in order to estimate the persistence of anti-trichomonas antibodies after eradication of protozoan infection by ELISA assay and western blotting. Results: Serological follow-up by ELISA showed the trending line of sera T. vaginalis IgG antibody going down after 4-5 months in the group of recovered patients; while those from the unrecovered/re-infection patients kept the high level of IgG antibody, a marker of infection persistence. Results from Western blot analysis showed very good correlation with those from ELISA assay and clinical symptoms during the course of follow-up periods. There were persistence of antibody to T. vaginalis antigen weighted 84 -115kDa in men partners and recovered patients. Conclusions: Host immunological response in trichomoniasis patients were antibody IgG, and dependent on the individual reaction. The titer of IgG decrease during the recovery time and become normal after 4-5 months. However, there were the persistence of antibody to T. vaginalis antigen 84 -115kDa in men partners and recovered patients. Key word: T. vaginalis, ELISA, Western blot

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