Tóm tắt

Background: Finding an efficient tool to identify and characterize the bioactive principles from the fruit peel of Calophyllum inophyllum L. by determining of calophyllolide, together with two phytosterols namely -sitosterol and stigmasterol by using pressurized liquid extraction (PLE) followed by gas chromatography­ mass spectrometry (GC-MS) in avoiding the tedious and time consuming methods of extraction, isolation, purification and structural elucidation of constituents. Material and methods: The fruit peels of Calophyllum inophyllum L. were obtained from Ben Tre Province-Vietnam, collected in 2009, identified by the botanic specialist. The analytes were extracted by PLE using several solvents/solvent syste ms. The extracts were tested for antimicrobial activities to determine the solvent(s) of choice. The GC-MS method was validated according to ICH guidelines. Results: The proposed method was selective against mass spectral deconvoluting software AMDIS32. The calibration curves obtained from the base peak area, was linear over the range of 3.19-51 µg.mL-1. The RSD of intra-and-inter-day precision variations were less than 2.1% and the mean recovery was (99.53 ± 2.05)% (RSD = 2.06%). The identification of calophyllolide was strictly confirmed using FDA and EU specifications whereas those of stigmasterol and -sitosterol were done using the NIST database. The quantitative results were: 0.0255 ± 0.0005%(m/m) for PLE extract and 0.025 ± 0.0005% (m/m) for soxhlet extract. Conclusion: For the first time in literature, the finding of calophyllolide together with stigmasterol and -sit oste rol, the phytosterols in Calophyllum inophyllum L. fruit peel using PLE followed by GC-MS. This has proved that this waste is a promising material as studies showed that calophyllolide is the biologically active component which possesses an antimicrobial and cytotoxic activities. 

Từ khoá

Toàn văn

PDF

Trong số này