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Background: Increased consumption of fresh vegetables that are high in polyphenols has been associated with a reduced risk of oxidative stress-induced disease. Spinacia oleracea is a good source of the bioflavonoid quercetin with many other flavonoids which exhibit anti-oxidant activity. With the purpose of creating a database to precisely identify this species to avoid confusion in the process of collecting samples of medicinal plants as well as to gradually build indicators as a basis for later research, we studied the characteristics of the leaves of Spinacia oleracea including: Microsurgery of leaf, powder characteristics of leaf, in order to standardized herbal Spinacia o/eracea. Materials and Methods: Leaves of Spinacia oleracea. Samples were collected in Hue city in March of 2016. Results: Powder characteristics of leaf: fiber bundles, starch, colored pieces, pieces of vascular, pieces of epidermis carrying stomata. Microsurgery of leaf: Section of leaves nerve has protruding upper and lower nerve. From outside to inside: epidermis, collenchyma cells, parenchyma cells. Next is phloem and xylem. The center of leaves nerve is usually empty. Leaves blade is characterized by palisade mesophyll cells and spongy mesophyll cells. Conclusion: These findings contribute to the testing and identifying standards of herbal Spinacia o/eracea, in order to standardize this herbal.

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